Sean McGinlay and Natalie King of Glasgow's Hilton Grosvenor hotel measured their pancake tower at 29.5 inches (75cm) - beating the current title by 0.4in.
The chefs mixed about 100 eggs, more than 17 pints of milk, 11lbs of flour and 6.6lbs of butter for the challenge, a hotel spokeswoman said.
The hotel's general manager Stuart Nelson said: 'It was a bit shaky towards the end but somehow we managed to pull it off.
'Special thanks must go to Sean McGinlay and the kitchen team, who have spent a great deal of time this week perfecting their pancake recipes and stacking techniques.'
According to Guinness World Records, the current official record stands at 29.1in (74cm), achieved in Slovenia in August 2008.
That 672-pancake stack took 22 hours to build.